DNA
Part:BBa_K3860001:Design
Designed by: Marius Schnutenhaus Group: iGEM21_RUBochum (2021-09-30)
αS2-casein from Bos taurus codon optimized for P. pastoris and secretion signal
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 809
Illegal BamHI site found at 890
Illegal XhoI site found at 244 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The αS2-casein from Bos taurus was optimized for P. pastoris. For secretion the alpha secretion signal was added.
Source
The genomic sequence of Bos taurus